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  • [Effect of exogenous fatty acids on the growth and production of exopolysaccharides of obligately methylotrophic bacterium Methylophilus quaylei].

[Effect of exogenous fatty acids on the growth and production of exopolysaccharides of obligately methylotrophic bacterium Methylophilus quaylei].

Prikladnaia biokhimiia i mikrobiologiia (2012-05-17)
S A M Otman, A B Pshenichnikova, V I Shvets
ABSTRACT

Accelerating growth and increasing exopolysaccharide production in obligate methylotrophic bacterium Methylophilus quaylei were observed in the presence of C12-C18 fatty acids added to the growth media. Sodium oleate was the best growth factor. Based on data on the composition of the free fatty acids fraction in the cells and the values of the zeta-potential and fluorescence anisotropy of whole cells, we suggested that fatty acids were incorporated in the outer membrane of M. quaylei.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Sodium oleate, ≥82% (fatty acids), powder
Sigma-Aldrich
Sodium oleate, ≥99%
Sigma-Aldrich
Sodium oleate, ≥95% (capillary GC)