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  • Bioassay-guided isolation and identification of desacetylmicrocolin B from Lyngbya cf. polychroa.

Bioassay-guided isolation and identification of desacetylmicrocolin B from Lyngbya cf. polychroa.

Planta medica (2009-05-12)
Theresa Meickle, Susan Matthew, Cliff Ross, Hendrik Luesch, Valerie Paul
ABSTRACT

Bioassay-guided fractionation of a non-polar extract of Lyngbya cf. polychroa resulted in the isolation of the cytotoxic desacetylmicrocolin B (1) as well as the known compounds microcolins A (2) and B (3). Compound 1 was found to inhibit the growth of HT-29 colorectal adenocarcinoma and IMR-32 neuroblastoma cells with half maximal inhibitory concentration (IC(50)) values of 14 nM for both cancer cell types. Microcolins A and B were found to have little activity against two strains of the marine fungus Dendryphiella salina with LD(50) values above 200 microg/mL. Compounds 1, 2, and 3 were obtained by reverse-phase chromatography and their structures were determined by NMR and MS. In this paper we report the isolation, identification, and biological activity of 1.

MATERIALS
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Sigma-Aldrich
Amphotericin B solution, 250 μg/mL in deionized water, 0.1 μm filtered, BioReagent, suitable for cell culture
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