Determination of benzotriazole and benzothiazole derivatives in marketed fish by double-vortex-ultrasonic assisted matrix solid-phase dispersion and ultrahigh-performance liquid chromatography-high resolution mass spectrometry.

Benzotriazoles (BTRs) and benzothiazoles (BTs) are two groups of emerging concern and high production volume contaminants. Via the biomagnification of the food web, they could jeopardize human health. In this work, rapid determining the presence of five BTRs and two BTs in marketed fish was performed by a novel double-vortex-ultrasonic assisted matrix solid-phase dispersion (DVUA-MSPD) and UHPLC-electrospray ionization (+)-quadrupole time-of-flight mass spectrometry detection. Unlike traditional MSPD, we simplified the method without the use of mortar/pestle and SPE-column procedures. The DVUA-MSPD factors were screened by a multilevel categorical design, and then optimized by Box-Behnken Design plus with response surface methodology. The limits of quantification were 0.15-2 ng g-1 (dry weight). The satisfactory average recovery ranged from 70% to 93% with RSDs less than 9%. The developed method was successfully applied for the rapid determination of selected BTRs and BTs in fish samples at trace-level.