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Merck

Innovative in vitro method to study ventilator induced lung injury.

ALTEX (2019-06-19)
Jon P Joelsson, Iwona T Myszor, Ari J Arason, Saevar Ingthorsson, Paulina Cherek, Gabriel S Windels, Kristjan Leosson, Gudmundur H Gudmundsson, Thorarinn Gudjonsson, Sigurbergur Karason
ABSTRACT

Mechanical ventilation (MV) is a life-saving therapy for critically ill patients, alleviating the work of breathing and supporting adequate gas exchange. However, MV can cause ventilator induced lung injury (VILI) by baro/volu- and atelectrauma, even lead to acute respiratory distress syndrome (ARDS), and substantially augment mortality. There is a need for specific biomarkers and novel research platforms for VILI/ARDS research to study these detrimental disorders and seek ways to avoid or prevent them. Previous in vitro studies on bronchial epithelium, cultured in air-liquid interface (ALI) conditions, have generally utilized static or constant pressure.  We have developed a Cyclical Pressure ALI Device (CPAD) that enables cyclical stress on ALI cultured human bronchial cells, with the aim of mimicking the effects of MV. Using CPAD we were able to analyze differentially expressed VILI/ARDS and innate immunity associated genes along with increased expression of associated proteins. CPAD provides an easy and accessible way to analyze functional and phenotypic changes that occur during VILI and may provide a platform for future drug testing.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-Goat IgG (whole molecule)–Peroxidase antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
Sigma-Aldrich
Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat, affinity isolated antibody
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Retinoic acid, ≥98% (HPLC), powder
Sigma-Aldrich
Collagen from human placenta, Bornstein and Traub Type IV, powder