Skip to Content
Merck
  • ELISA detection of semicarbazide based on a fast sample pretreatment method.

ELISA detection of semicarbazide based on a fast sample pretreatment method.

Chemical communications (Cambridge, England) (2013-06-04)
Zhiyuan Fang, Baishan Jiang, Wei Wu, Zhicheng Xiang, Chuanyan Ouyang, Tulong Huang, Junhua Chen, Lingwen Zeng
ABSTRACT

A direct ELISA was established for the fast detection of semicarbazide (SEM) using a novel biotin derivative. Without a tedious extraction procedure, as low as 0.07 Ī¼g L(-1) of SEM could be detected reproducibly. This assay has better recovery and accuracy than competitive ELISA.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
1-Biotinyl-3,6-dioxa-8-octaneamine hydrochloride, ≥95%