R8257
Mlu I from Micrococcus luteus (lysodeikticus)
buffered aqueous glycerol solution
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
form
buffered aqueous glycerol solution
concentration
10,000 units/mL
shipped in
wet ice
storage temp.
−20°C
Specificity
Recognition sequence: 5′-A/CGCGT-3′
Ligation and recutting results: After 2-10-fold Mlu I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Partially inactivated at 65 °C for 10 minutes.
Ligation and recutting results: After 2-10-fold Mlu I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Partially inactivated at 65 °C for 10 minutes.
Other Notes
Supplied with 10x Restriction Endonuclease Buffer SH (B3657).
Caution
Comment: Mlu I will only partially cleave DNA isolated from E. coli strains that have the dam methylase (dam+ strains).
Physical form
Solution in 20 mM Tris-HCl, pH 8.0, 0.1 mM EDTA, 250 mM KCl, 10 mM 2-mercaptoethanol, 50% glycerol (v/v), 0.2% Triton X-100 (v/v) at 4°C
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New restriction endonucleases from Flavobacterium okeanokoites (FokI) and Micrococcus luteus (MluI).
H Sugisaki et al.
Gene, 16(1-3), 73-78 (1981-12-01)
Two new restriction endonucleases have been isolated from Flavobacterium okeanokoites IFO12536 and Micrococcus luteus IFO12992 and named FokI and MluI, respectively. Based on analysis of the sequences around the restriction sites, the recognition sequences and cleavage sites of these endonucleases
Jaroslav Jelinek et al.
Epigenetics, 7(12), 1368-1378 (2012-10-19)
Genome wide analysis of DNA methylation provides important information in a variety of diseases, including cancer. Here, we describe a simple method, Digital Restriction Enzyme Analysis of Methylation (DREAM), based on next generation sequencing analysis of methylation-specific signatures created by
Cong Zhu et al.
Nucleic acids research, 41(4), 2455-2465 (2013-01-11)
Zinc-finger nucleases (ZFNs) have been used for genome engineering in a wide variety of organisms; however, it remains challenging to design effective ZFNs for many genomic sequences using publicly available zinc-finger modules. This limitation is in part because of potential
Saqib H Ansari et al.
Journal of pediatric hematology/oncology, 35(4), e153-e156 (2013-02-08)
β-thalassemia is characterized by impaired β-chain synthesis leading to ineffective erythropoiesis, severe anemia, and a need for blood transfusion. Presence of Xmn I polymorphism (-158 C-T nucleotide change) in γ-globin gene is associated with a higher fetal hemoglobin and a
Amal M El-Shehaby et al.
Scandinavian journal of clinical and laboratory investigation, 73(1), 75-81 (2012-12-04)
Left ventricular hypertrophy (LVH) is a common manifestation of cardiovascular disease and has an important prognostic value in patients with end-stage renal disease (ESRD). Vitamin D receptor (VDR) has been intensively investigated, and one of these (BsmI) already has been
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