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MAK417

Sigma-Aldrich

Glycogen Phosphorylase Colorimetric Assay Kit

sufficient for 100 colorimetric tests

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About This Item

UNSPSC Code:
12161503

detection method

colorimetric

relevant disease(s)

cancer

storage temp.

−20°C

General description

Glycogen phosphorylase catalyzes the rate-limiting step in glycogenolysis using glycogen and inorganic phosphate to produce glucose-1-phosphate (G1P). In mammals, glycogen phosphorylase is abundant in muscle, liver, and brain tissues. There are two forms of glycogen phosphorylase, namely glycogen phosphorylase a and b forms. Glycogen phosphorylase a is the highly active form whereas glycogen phosphorylase b has only limited activity. Glycogen phosphorylase is a clinically significant enzyme as its mutations are associated with different glycogen storage diseases in muscle and liver. In addition, this enzyme has been suggested as a biomarker for gastric cancer and its inhibition has been tested in treating type 2 diabetes.

Features and Benefits

Glycogen Phosphorylase Assay Kit:
  • Detects as low as 10 mU in a variety of sample types.

Suitability

The kit is suitable for the measurement of glycogen phosphorylase activity in cell culture and animal tissues (heart, liver, kidney, muscle, etc.) as well as the analysis of glycogen phosphorylase kinetics, inhibition or activation.

Principle

The Glycogen Phosphorylase Colorimetric Activity Assay Kit is based on the detection of G1P by a set of enzymatic reactions to generate a colored product with a strong absorbance at A450 nm. The A450 nm signal is directly proportional to the glycogen phosphorylase activity. The kit is a rapid, sensitive and convenient tool for detecting glycogen phosphorylase activity.

Pictograms

Health hazardCorrosion

Signal Word

Danger

Hazard Statements

Hazard Classifications

Aquatic Chronic 3 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B

Storage Class Code

8A - Combustible corrosive hazardous materials

WGK

WGK 3


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