PLD35

GenElute^™ Plasmid Midiprep Kit

sufficient for 35 purifications

• Synonym(s): Gen Elute, GenElute^™ Plasmid Kit, plasmid extraction kit, plasmid isolation kit, plasmid purification kit
• NACRES: NA.52
• UNSPSC Code: 41105501

Properties

• usage: sufficient for 35 purifications
• Quality Level: 200
• greener alternative product characteristics: Inherently Safer Chemistry for Accident Prevention; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• technique(s): DNA purification: suitable
• test parameters: : ~45 min kit run time, sample volume: 5-40 mL bacterial culture
• greener alternative category: Aligned,
• storage temp.: 15-25°C

Description

General description

Click here to view the kit protocol

The GenElute Plasmid Midiprep Kit offers a simple, rapid, cost-effective method for isolating plasmid DNA from recombinant E. coli cultures. By combining silica- binding technology and the convenience of a spin column format, up to 300 μg of plasmid DNA can be recovered from 20-40 ml of Luria Broth (LB) in about 45 minutes. Note that actual yield and optimal volume of culture to use depend on the plasmid and the culture medium.

An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto silica in the presence of high salts. Contaminants are then removed by a spin-wash step. Finally, the bound DNA is eluted in water or Tris-EDTA buffer.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Application

The DNA is ready for immediate use in such downstream applications as restriction enzyme digestion, cloning, PCR, transcription and automated sequencing.

The recovered plasmid DNA is predominately in its supercoiled form. There is no visual evidence of genomic DNA or RNA contamination detected by agarose gel electrophoresis. The DNA is ready for immediate use in downstream applications such as restriction digestion, ligation, sequencing, PCR, and transfection.

Biochem/physiol Actions

Bacterial cells are harvested via centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorbtion of DNA onto silca in the presence of high salts. Contaminants are then removed by a simple wash step. Finally, the bound DNA is eluted in water or Tris-EDTA buffer. The recovered plasmid DNA is predominately in its supercoiled form. There is no visual evidence of genomic DNA or RNA contamination.

Other Notes

GenElute Plasmid Midi- and Maxiprep Kits offer a simple, rapid and cost-effective method for isolating plasmid DNA from E. coli cultures. These kits combine silica-based membrane technology with the convenience of a spin column format and deliver up to 350 μg (Midi) and 1.2 mg (Maxi) yield of high-copy plasmid DNA.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

• R1149

  Resuspension Solution, Add RNase A before use. After addition of RNAse, store at 2-8ºC. )

  SDS
• C2112

  Column Preparation Solution

  SDS
• R6148

  RNase A solution

  SDS
• L1912

  Lysis Solution

  SDS

Safety Information

• pictograms: GHS02,GHS08,GHS05,GHS07
• signalword: Danger
• hcodes: H226,H290,H302,H315,H319,H334,H336
• pcodes: P210 - P233 - P234 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Central nervous system
• Storage Class: 3 - Flammable liquids
• wgk: WGK 3