SEQR
SeqPlex RNA Amplification Kit
For use with high throughput sequencing technologies
Synonym(s):
WGA kit, Whole transcriptome amplification
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10 REACTIONS
MXP 20,413.00
50 REACTIONS
MXP 92,791.00
500 REACTIONS
MXP 540,790.00
MXP 20,413.00
Estimated to ship onApril 07, 2025
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10 REACTIONS
MXP 20,413.00
50 REACTIONS
MXP 92,791.00
500 REACTIONS
MXP 540,790.00
About This Item
UNSPSC Code:
41121800
NACRES:
NA.55
MXP 20,413.00
Estimated to ship onApril 07, 2025
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This Item | SEQXE | WTA2 | WTA1 |
|---|---|---|---|
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
General description
The SeqPlex™ RNA Amplification Kit for whole transcriptome amplification (WTA) is designed to facilitate next-generation sequencing (NGS) from small quantities or from degraded/highly fragmented RNA (e.g. RNA from formalin-fixed paraffin-embedded (FFPE) tissue samples). The SeqPlex kit allows the user to pre-amplify these and other small quantity/highly fragmented RNA samples for input into a NGS workflow. It also facilitates the amplification of non-polyA tailed RNA isolated from tissue, cultured cells, formalin-fixed samples, or serum while maintaining patterns of differential expression found in the unamplified sample.
Features and Benefits
- Low quantities of total RNA random priming technology amplifies fragmented or intact RNA from all sources including FFPE and RIP.
- Semi-degenerate library primer design for more complete transcriptome coverage and efficient priming.
- No need to fragment DNA before sequencing.
- Amplifies ds-cDNA in 8 hours or less.
- Compatible with all next generation sequencing platforms except Pacific Bioscience.
Other Notes
SEQR-500RXN is manufactured on-demand. Contact us for more information.
Legal Information
SeqPlex is a trademark of Sigma-Aldrich Co. LLC
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Description
Pricing
Storage Class Code
11 - Combustible Solids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Protocols
The SeqPlex RNA Amplification kit provides a method for amplification of total RNA or isolated mRNA prior to entry into the workflows of the commonly used deep sequencing platforms.
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