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M0404

Sigma-Aldrich

Murashige and Skoog Basal Medium

powder, suitable for plant cell culture, with Gamborg′s vitamins

Synonym(s):

MS0 Medium, MSO Medium, Murashige and Skoog (Gamborg Modified) Basal Media, MS Basal Medium

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1 L
MXP 364.00
10 L
MXP 1,058.00

MXP 364.00


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1 L
MXP 364.00
10 L
MXP 1,058.00

About This Item

MDL number:
UNSPSC Code:
12352207
NACRES:
NA.72

MXP 364.00


In StockDetails


Request a Bulk Order

Quality Level

form

powder

technique(s)

cell culture | plant: suitable

application(s)

agriculture

shipped in

ambient

storage temp.

2-8°C

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Application

Murashige and Skoog Basal Medium has been used:
  • to germinate the seeds for seedling growth and germination studies[1]
  • in the suspension culture of Nicotiana plumbaginifolia for transformation[2]
  • in the root culture of hairy clone A. artemisiifolia T4[3]

Formula variant

With the macro- and micronutrients as described by Murashige and Skoog (1962) and the vitamins as described by Gamborg, et al. (1968).

Media Formulation

Quantity

Formulated to contain 4.4 grams of powder per liter of medium.

Preparation Note

Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient, micronutrient and vitamin mixtures.
Murashige and Skoog medium (M0404) contains the macro- and micronutrients of M&S basal medium as described by Gamborg, et al (1968). It can be supplemented with organics and with auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.

Pictograms

Flame over circleExclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Ox. Sol. 3

Storage Class Code

5.1B - Oxidizing hazardous materials

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Ken-Der Wang et al.
The Plant cell, 32(1), 166-185 (2019-11-07)
Multiple long-distance signals have been identified for pathogen-induced systemic acquired resistance, but mobile signals for symbiont-induced systemic resistance (ISR) are less well understood. We used ISR-positive and -negative mutants of maize (Zea mays) and the beneficial fungus Trichoderma virens and
Brenden Hurley et al.
PloS one, 9(12), e114921-e114921 (2014-12-17)
Pseudomonas syringae subverts plant immune signalling through injection of type III secreted effectors (T3SE) into host cells. The T3SE HopF2 can disable Arabidopsis immunity through Its ADP-ribosyltransferase activity. Proteomic analysis of HopF2 interacting proteins identified a protein complex containing ATPases
Crescencio Bazaldúa et al.
PloS one, 14(9), e0222464-e0222464 (2019-09-13)
Ten Hyptis suaveolens hairy root lines were established by infecting nodal explants with K599+pGus-GFP+ and ATCC15834+pTDT strains from Agrobacterium rhizogenes. Genetic transformation was confirmed by epifluorescence and plagiotropic hairy root growth in absence of growth regulators. Cytotoxicity was determined using
Identification of a Golgi-localised GRIP domain protein from Arabidopsis thaliana
Gilson PR, et al.
Planta, 219(6), 1050-1056 (2004)
Itai Bloch et al.
Plant biotechnology journal, 19(9), 1785-1797 (2021-03-28)
The synthesis of essential amino acids in plants is pivotal for their viability and growth, and these cellular pathways are therefore targeted for the discovery of new molecules for weed control. Herein, we describe the discovery and design of small

Questions

1–2 of 2 Questions  
  1. What is electric conductivity of Murashige-Skoog culture solution?

    1 answer
    1. The Murashige and Skoog media products have not been tested for conductivity.

      Helpful?

  2. I would like to know if there is any problem in irradiating with UV light, media culture prepared with the medium.

    1 answer
    1. Testing protocols for plant culture media include sterilization by autoclaving. Heat-labile components are filter-sterilized and added after autoclaving, prior to asceptic dispensing to sterile vessels. UV sterilization has not been investigated as a sterilizing technique for culture media products, although this does appear to be growing in popularity. Keep in mind that UV would not melt agar used in solid media formats. Autoclaving or boiling would still be required. Please see the link below for a publication that may offer additional information:
      https://pubmed.ncbi.nlm.nih.gov/25044686/#:~:text=Ultraviolet%20(UV)%20irradiation%20is%20a,sterilization%20of%20cell%20culture%20media.

      Helpful?

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