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  • Complex activity and short-term plasticity of human cerebral organoids reciprocally connected with axons.

Complex activity and short-term plasticity of human cerebral organoids reciprocally connected with axons.

Nature communications (2024-04-11)
Tatsuya Osaki, Tomoya Duenki, Siu Yu A Chow, Yasuhiro Ikegami, Romain Beaubois, Timothée Levi, Nao Nakagawa-Tamagawa, Yoji Hirano, Yoshiho Ikeuchi
ABSTRACT

An inter-regional cortical tract is one of the most fundamental architectural motifs that integrates neural circuits to orchestrate and generate complex functions of the human brain. To understand the mechanistic significance of inter-regional projections on development of neural circuits, we investigated an in vitro neural tissue model for inter-regional connections, in which two cerebral organoids are connected with a bundle of reciprocally extended axons. The connected organoids produced more complex and intense oscillatory activity than conventional or directly fused cerebral organoids, suggesting the inter-organoid axonal connections enhance and support the complex network activity. In addition, optogenetic stimulation of the inter-organoid axon bundles could entrain the activity of the organoids and induce robust short-term plasticity of the macroscopic circuit. These results demonstrated that the projection axons could serve as a structural hub that boosts functionality of the organoid-circuits. This model could contribute to further investigation on development and functions of macroscopic neuronal circuits in vitro.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-Tau-1 Antibody, clone PC1C6, clone PC1C6, Chemicon®, from mouse
Sigma-Aldrich
Anti-Synapsin I Antibody, serum, Chemicon®
Sigma-Aldrich
Anti-β-Tubulin III Antibody, clone 5H16, ZooMAb® Rabbit Monoclonal, recombinant, expressed in HEK 293 cells