Skip to Content
Merck
  • Accelerated identification of Staphylococcus aureus from blood cultures by a modified fluorescence in situ hybridization procedure.

Accelerated identification of Staphylococcus aureus from blood cultures by a modified fluorescence in situ hybridization procedure.

Journal of medical microbiology (2009-10-03)
Sven Poppert, Melanie Riecker, Nele Wellinghausen, Hagen Frickmann, Andreas Essig
ABSTRACT

This study evaluated fluorescence in situ hybridization (FISH) for rapid identification of Staphylococcus aureus and coagulase-negative staphylococci (CoNS) directly from blood cultures. Initially, 360 blood cultures containing Gram-positive cocci were investigated by a previously described microwave-FISH procedure: 44/49 (89.8 %) S. aureus and 298/299 (99.7 %) CoNS were correctly identified. Because FISH proved useful and reliable but handling was found to be inconvenient, the method was modified by employing a recently developed slide chamber. This reduced the time required from 60 to 30 min. The simplified execution allowed integration of the method into the workflow of a routine laboratory without difficulty. The modified method proved to be highly reliable, identifying 37/37 (100 %) S. aureus and 169/172 (98.2 %) CoNS directly from blood cultures.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Escherichia coli FISH probe -ATTO488, Probe for fluorescence in situ hybridization (FISH), 20µM in water
Sigma-Aldrich
Enterococcus faecium FISH probe – ATTO 488, Probe for fluorescence in situ hybridization (FISH), 20µM in water
Sigma-Aldrich
Akkermansia muciniphila FISH probe-ATTO488, Probe for fluorescence in situ hybridization (FISH), 20µM in water
Sigma-Aldrich
Enterococcus faecium FISH probe - Cy3, Probe for fluorescence in situ hybridization (FISH), 20µM in water
Sigma-Aldrich
Alphaproteobacteria FISH probe-Cy3, Probe for fluorescence in situ hybridization (FISH), 20 μM in water