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  • The lutheran/basal cell adhesion molecule promotes tumor cell migration by modulating integrin-mediated cell attachment to laminin-511 protein.

The lutheran/basal cell adhesion molecule promotes tumor cell migration by modulating integrin-mediated cell attachment to laminin-511 protein.

The Journal of biological chemistry (2013-09-17)
Yamato Kikkawa, Takaho Ogawa, Ryo Sudo, Yuji Yamada, Fumihiko Katagiri, Kentaro Hozumi, Motoyoshi Nomizu, Jeffrey H Miner
ABSTRACT

Cell-matrix interactions are critical for tumor cell migration. Lutheran (Lu), also known as basal cell adhesion molecule (B-CAM), competes with integrins for binding to laminin α5, a subunit of LM-511, a major component of basement membranes. Here we show that the preferential binding of Lu/B-CAM to laminin α5 promotes tumor cell migration. The attachment of Lu/B-CAM transfectants to LM-511 was slightly weaker than that of control cells, and this was because Lu/B-CAM disturbed integrin binding to laminin α5. Lu/B-CAM induced a spindle cell shape with pseudopods and promoted cell migration on LM-511. In addition, blocking with an anti-Lu/B-CAM antibody led to a flat cell shape and inhibited migration on LM-511, similar to the effects of an activating integrin β1 antibody. We conclude that tumor cell migration on LM-511 requires that Lu/B-CAM competitively modulates cell attachment through integrins. We suggest that this competitive interaction is involved in a balance between static and migratory cell behaviors.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-Integrin β1 Antibody, clone 6S6, clone 6S6, Chemicon®, from mouse
Sigma-Aldrich
Anti-Integrin β4 Antibody, clone ASC-3, clone ASC-3, Chemicon®, from mouse