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A8062

Sigma-Aldrich

Anti-Goat/Sheep IgG−Alkaline Phosphatase antibody, Mouse monoclonal

clone GT-34, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-Goat/Sheep IgG, Monoclonal Anti-Goat/Sheep IgG–Alkaline Phosphatase antibody produced in mouse

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

biological source

mouse

conjugate

alkaline phosphatase conjugate

antibody form

purified from hybridoma cell culture

antibody product type

secondary antibodies

clone

GT-34, monoclonal

form

buffered aqueous glycerol solution

species reactivity

sheep, goat, bovine

should not react with

rat, canine, mouse, rabbit, guinea pig, chicken, horse, pig, feline, human

technique(s)

direct ELISA: 1:50,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40
western blot (chemiluminescent): 1:150,000 using total cell extract of chicken fibroblasts (10-20 μg per well)

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

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General description

IgGs are glycoprotein antibodies that modulate several immune responses. Primary goat against target proteins are often used in various research applications. Thus, secondary anti-goat IgGs can be useful tools for the analysis of target proteins. However, secondary antibodies may lack species specificity as they may bind to non-related Igs in the test preparation. This may lead to increased levels of background staining. Hence, use of monoclonals can effectively reduce background staining and increase the specificity of immunoassays.
Monoclonal Anti-Goat/Sheep IgG-Alkaline Phosphatase recognizes goat and sheep IgGs. No cross-reactivity is observed with human IgGs or IgGs derived from the following species: guinea pig, rat, horse, dog, chicken, pig, mouse, rabbit, and cat.
The antibody reacts with an epitope located on the heavy chain of goat IgG1 and IgG2.

Immunogen

Purified goat IgG

Application

Mouse monoclonal anti-goat/sheep IgG-alkaline phosphatase antibody has been used as a secondary antibody for ELISA assays at 1:4,000 and 1:15,210 dilutions. The product can also be used for IHC (1:40) and western blot applications (1:150,000).
The level of IL-5 in HPAEC conditioned medium was determined by ELISA using alkaline phosphatase-conjugated monoclonal mouse anti-goat/sheep IgG (GT-34) as the secondary antibody.

Physical form

Solution in 0.05 M Tris buffer pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin, 50% glycerol, and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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