94581

Hirudin recombinant from yeast

lyophilized powder, white, >1500 U/vial

• CAS Number: 8001-27-2
• EC Number: 232-279-1
• MDL number: MFCD00803952
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• biological source: synthetic; yeast (Pichia pastoris)
• form: lyophilized powder
• specific activity: >1500 U/vial
• mol wt: M_r ~6963.5
• color: white
• solubility: 0.1 M Tris, 0.2 M NaCl, pH 8.4: 1 vial/mL, clear, colorless
• storage temp.: −20°C

Description

Application

Recombinant hirudin from yeast has been used in mannan-binding lectin serine protease 2 (MASP2) activation assay. It has also been used in pre-treating the nanosheets in phosphate buffered saline (PBS) for platelet adhesion assay.

Biochem/physiol Actions

Recombinant hirudin appears to be antigenic. Independent studies have found that 50-70% of patients treated for several days with recombinant hirudin develop at least one hirudin antibody isotype. The antibodies reduce the anticoagulant effect of hirudin, but prolong its plasma half-life by reducing renal filtration.

The anticoagulant, hirudin, is the most potent natural inhibitor of both soluble and clot-bound thrombin. It binds thrombin with high affinity, covering more than 20% of its surface area and occluding both the active site and exosite I (fibrinogen and PAR recognition site). Hirudin blocks thrombus growth and platelet activation. It is not metabolized in the bloodstream of humans and is eliminated unchanged via kidney filtration.

Unit Definition

1 U corresponds to 1 ATU (antithrombin unit; 1 ATU is defined as the amount of inhibitor that neutralizes 1 NIH unit of thrombin).

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves