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1.12374

Sigma-Aldrich

Thrombin

from bovine plasma, 50 NIH units/mg dry wt

Synonym(s):

Thrombin, Coagulation factor IIa, Fibrinogenase

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About This Item

CAS Number:
UNSPSC Code:
12352204
EC Index Number:
232-648-7
NACRES:
NA.25

biological source

bovine plasma

Quality Level

form

solid

specific activity

50 NIH units/mg dry wt

storage temp.

2-8°C

General description

Thrombin, a glycosylated sodium-activated type II enzyme, is also called coagulation factor IIa. It contains two anion-binding exosites, ABE-I and ABE-II. Thrombin exists as α, β, and γ isoforms at different stages of its generation. Bovine a-thrombin consists of a light chain (A chain) and a heavy chain (B chain). These two chains are joined by one disulfide bond.

Application

Thrombin has been used in flow cytometry analysis to investigate the basal activation level of circulating platelets in mice. It has also been used to coagulate the plasma and allow the organotypic slice to adhere to the coverslip during culturing.

Biochem/physiol Actions

Thrombin is a serine protease critical in the blood clotting process and activates clotting factors V, VIII, XI, and XII. It mediates the conversion of inactive plasma protein fibrinogen (factor I) into biologically active fibrin. Thrombin acts as a pro-coagulant and promotes platelet aggregation. It can also produce anti-coagulant effects. It is used to treat bleeding from capillaries and small venules. During vascular injury, thrombin is crucial for controlling the macrophages, neutrophils, monocytes, SMC, and dendritic cells.

Analysis Note

Appearance (colour): almost white
Appearance (description): powder
Activity (fibrinogen; pH 7.6; 37 °C): ≥ 50 U/mg

Pictograms

Exclamation markHealth hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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