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Sigma-Aldrich

Annexin V-Cy3™ Apoptosis Detection Kit

Synonym(s):

Red fluorescent Annexin V apoptosis assay

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.32

usage

sufficient for 200 tests

Quality Level

200

packaging

pkg of 1 kit

technique(s)

flow cytometry: suitable

application(s)

cell analysis
detection

detection method

fluorometric

shipped in

wet ice

storage temp.

2-8°C

Features and Benefits

Detects apoptosis earlier in the process than DNA-based assays such as TUNEL
  • Rapid labeling of cells. Cell staining takes only 10 minutes.
  • No cell fixation or processing required, reducing the detection time and allowing the cells to be used for further study.
  • 6-Carboxyfluorescein diacetate secondary dye is included with the kit to differentiate apoptotic cells from viable and necrotic cells.

Principle

Sigma′s Annexin V-Cy3 kit allows detection of annexin V bound to apoptotic cells by fluorescence microscopy. The Annexin V-Cy3 kit uses the dye Cy3.18 as the fluorochrome conjugated with annexin V. By microscopy, Cy3.18 fluoresces more brightly than the FITC conjugate. The kit includes the non-fluorescent compound 6-carboxyfluorescein diacetate (6-CFDA), which enters the cell and is hydrolyzed by the esterases present in living cells to the fluorescent compound 6-carboxyfluorescein, indicating that the cells are viable. This combination allows the differentiation among early apoptotic cells (annexin V positive, 6-CFDA positive), necrotic cells (annexin V positive, 6-CFDA negative), and viable cells (annexin V negative, 6-CFDA positive).

Legal Information

Cy3 is a trademark of Cytiva

Kit Components Only

Product No.
Description
  • Annexin V Cy3.18 Conjugate from human placenta, buffered aqueous solution 10 μg

Kit Components Also Available Separately

Product No.
Description
SDS
  • C50416-Carboxyfluorescein diacetate 10 mgSDS

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Christo Paul Preethy et al.
Journal of pharmacology & pharmacotherapeutics, 3(1), 26-34 (2012-03-01)
To find the efficacy of serial extracts of Anisomeles malabarica in inhibiting proliferation of and inducing apoptosis in human cervical cancer cells, SiHa and ME 180, that are HPV 16-positive. The whole plant was extracted in n-hexane, chloroform, ethyl acetate
Rajeev Kumar Pandey et al.
PloS one, 6(2), e17093-e17093 (2011-02-25)
Mycobacterium indicus pranii (MIP), also known as Mw, is a saprophytic, non-pathogenic strain of Mycobacterium and is commercially available as a heat-killed vaccine for leprosy and recently tuberculosis (TB) as part of MDT. In this study we provide evidence that
Kasturi Muthoosamy et al.
Scientific reports, 6, 32808-32808 (2016-09-07)
Metastasis of lung carcinoma to breast and vice versa accounts for one of the vast majority of cancer deaths. Synergistic treatments are proven to be the effective method to inhibit malignant cell proliferation. It is highly advantageous to use the
Mohamed Asik R et al.
Materials science & engineering. C, Materials for biological applications, 103, 109840-109840 (2019-07-28)
Development of novel approach for cancer therapy, sparing healthy normal cells overcoming the limitation of available therapies is of prime importance for cervical cancer treatment. Recently metal oxide based chemotherapeutics has emanated as a promising approach for cancer therapy. Hence
Andre F Steinert et al.
Arthritis research & therapy, 11(5), R148-R148 (2009-10-06)
The present study compares bone morphogenetic protein (BMP)-4 and BMP-2 gene transfer as agents of chondrogenesis and hypertrophy in human primary mesenchymal stem cells (MSCs) maintained as pellet cultures. Adenoviral vectors carrying cDNA encoding human BMP-4 (Ad.BMP-4) were constructed by
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Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

Apoptosis Assays

Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

Apoptosis Assays

Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

Apoptosis Assays

Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

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