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Key Documents

PCHT12H48

Millipore

Millicell® 12 well hanging cell culture inserts

pore size 0.4 μm, clear PET membrane, diam. 12 mm, Tissue Culture (TC)-treated surface, sterile

Synonym(s):

permeable inserts, tissue culture inserts, transparent membrane insert, imaging insert

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About This Item

UNSPSC Code:
41122107
NACRES:
NB.13

material

clear PET membrane
polystyrene housing

Quality Level

sterility

γ-irradiated
sterile

feature

4x106 pores/cm2 (pore density)

packaging

pack of 48 ea (individually blister packaged)

manufacturer/tradename

Millicell®

parameter

max. temp. at 37 °C

technique(s)

cell attachment: suitable
cell differentiation: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
permeability assay: suitable

H

15.5 mm

diam.

12 mm

insert maximum volume

0.8 mL

insert working volume

0.4 mL

membrane thickness

9-16 μm

size

12 wells

surface area

1.1 cm2

color

clear membrane
transparent membrane

pore size

0.4 μm pore size

binding type

Tissue Culture (TC)-treated surface

General description

Millicell® Cell Culture inserts with Polyethylene Terephthalate (PET) membrane are ideal for the growth and differentiation of various cell types. This individually sterile-packaged insert has an optically clear 0.4 μm membrane pore size and fits in most 12-well tissue culture plates.

Application

Barrier Assays
Cell Proliferation
Cell Surface Receptors
Co-Culture
Epithelial Cell Growth
Feeder Layers Fluorescent
Detection /Immunohistochemistry
In Vitro Fertilization
In Vitro Toxicology
Microbial Attachment
Phase Contrast Microscopy Polarized Protein Secretions
Polarized Uptake Transport/Permeability

Features and Benefits

  • Optically clear TC-treated PET membrane
  • Low pore density (4 million pores/cm2)
  • Compatible with most 12-well plates
  • Apical assist rim to prevent membrane puncture or disruption of cells
  • Easily access the basolateral side between the flanges

Legal Information

Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

How to set up barrier formation and permeability assays using cell culture inserts and TEER measurements.

How to set up barrier formation and permeability assays using cell culture inserts and TEER measurements.

How to set up barrier formation and permeability assays using cell culture inserts and TEER measurements.

How to set up barrier formation and permeability assays using cell culture inserts and TEER measurements.

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