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Key Documents

04-902

Sigma-Aldrich

Anti-HA Tag Antibody, clone DW2, rabbit monoclonal

culture supernatant, clone DW2, from rabbit

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.43

biological source

rabbit

Quality Level

antibody form

culture supernatant

antibody product type

primary antibodies

clone

DW2, monoclonal

species reactivity

vertebrates (common species)

technique(s)

western blot: suitable

isotype

IgG

shipped in

dry ice

target post-translational modification

unmodified

General description

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule that corresponds to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

Specificity

Reactivity with other species has not been confirmed.
Recognizes recombinant proteins containing the HA epitope tag. The apparent molecular weight is related to the tagged protein.

Immunogen

KLH-conjugated, synthetic peptide containing the sequence …CYPYDVPDY… derived from the influenza virus hemaglutinin (HA) protein.

Application

Anti-HA Tag Antibody, clone DW2 is an antibody against HA Tag for use in WB.
Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags
Western Blot Analysis: A 1:1,000-1:2,000 dilution of this lot detected HA-tagged WNT-1 protein expressed in HEK293 cells.

Quality

Western Blot Analysis
Lysates from non-transfected (lane 1) and HAtagged WNT-1 transfected (lane 2) HEK293 cells were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-HA-Tag, clone DW2 (1:1,000 dilution).
Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.

Arrow indicates the HA-tagged protein (~49 kDa). The apparent molecular weight is related to the tagged protein.

Target description

Various

Linkage

Replaces: 05-902

Physical form

Cultured supernatant in 0.05% sodium azide.

Storage and Stability

Stable for 1 year at -20°C from date of shipment. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
HA-tagged WNT-1 protein.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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