Twelve-hour PCR-based method for detection of Salmonella spp. in food.

Twelve-hour PCR-based method for detection of Salmonella spp. in food.

Applied and environmental microbiology (2001-02-07)

R Ferretti, I Mannazzu, L Cocolin, G Comi, F Clementi

ABSTRACT

A PCR-based method for the detection of Salmonella spp. in food was developed. The method, set up on typical salami from the Italian region of Marche, is sensitive and specific and shows excellent correlation with the conventional method of reference when naturally contaminated foods are analyzed. Moreover, it can be easily performed within a maximum of 12 h from food sampling, thus allowing prompt detection of Salmonella spp. in the food stocks analyzed.

MATERIALS

Product Number

Brand

Product Description

11218611910

Roche

DNA Molecular Weight Marker VI, DIG-labeled, solution, pkg of 500 μL (10 μg/ml)

11062590001

Roche

DNA Molecular Weight Marker VI, pkg of 50 μg (in 200 μl), solution