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  • The effects of local vanadium treatment on angiogenesis and chondrogenesis during fracture healing.

The effects of local vanadium treatment on angiogenesis and chondrogenesis during fracture healing.

Journal of orthopaedic research : official publication of the Orthopaedic Research Society (2012-06-02)
David N Paglia, Aaron Wey, Andrew G Park, Eric A Breitbart, Siddhant K Mehta, John D Bogden, Francis W Kemp, Joseph Benevenia, J Patrick O'Connor, Sheldon S Lin
ABSTRACT

This study quantified the effects of local intramedullary delivery of an organic vanadium salt, which may act as an insulin-mimetic on fracture healing. Using a BB Wistar rat femoral fracture model, local vanadyl acetylacetonate (VAC) was delivered to the fracture site and histomorphometry, mechanical testing, and immunohistochemistry were performed. Callus percent cartilage was 200% higher at day 7 (p < 0.05) and 88% higher at day 10 (p < 0.05) in the animals treated with 1.5 mg/kg of VAC. Callus percent mineralized tissue was 37% higher at day 14 (p < 0.05) and 31% higher at day 21 (p < 0.05) in the animals treated with 1.5 mg/kg of VAC. Maximum torque to failure was 104% and 154% higher at 4 weeks post-fracture (p < 0.05) for the healing femurs from the VAC-treated (1.5 and 3.0 mg/kg) animals. Animals treated with other VAC doses demonstrated increased mechanical parameters at 4 weeks (p < 0.05). Immunohistochemistry detected 62% more proliferating cells at days 7 (p < 0.05) and 94% more at day 10 (p < 0.05) in the animals treated with 1.5 mg/kg VAC. Results showed 100% more vascular endothelial growth factor-C (VEGF-C) positive cells and 80% more blood vessels at day 7 (p < 0.05) within the callus subperiosteal region of VAC-treated animals (1.5 mg/kg) compared to controls. The results suggest that local VAC treatment affects chondrogenesis and angiogenesis within the first 7-10 days post-fracture, which leads to enhanced mineralized tissue formation and accelerated fracture repair as early as 3-4 weeks post-fracture.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Manganese(III) acetylacetonate, technical grade
Sigma-Aldrich
Copper(II) acetylacetonate, 97%
Sigma-Aldrich
Copper(II) acetylacetonate, ≥99.9% trace metals basis
Sigma-Aldrich
Manganese(II) acetylacetonate
Sigma-Aldrich
Chromium(III) acetylacetonate, 97%