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P4957

Sigma-Aldrich

Poly-ʟ-Ornithine

synthetic, mol wt 30,000-70,000, liquid, 0.01%, suitable for cell culture

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352202
PubChem Substance ID:
NACRES:
NA.75

product name

Poly-L-ornithine solution, mol wt 30,000-70,000, 0.01%, sterile-filtered, BioReagent, suitable for cell culture

biological source

synthetic

sterility

sterile-filtered

product line

BioReagent

form

solution

mol wt

30,000-70,000

packaging

pkg of 50 mL

concentration

0.01%

technique(s)

cell culture | mammalian: suitable

surface coverage

4 μg/cm2

impurities

endotoxin, tested

shipped in

ambient

storage temp.

2-8°C

SMILES string

Br.NCCCC(N)C(O)=O

InChI

1S/C5H12N2O2.BrH/c6-3-1-2-4(7)5(8)9;/h4H,1-3,6-7H2,(H,8,9);1H

InChI key

GWRQMKDBBHFVIZ-UHFFFAOYSA-N

General description

Poly-L-ornithine is a positively charged synthetic amino acid polymer.

Application

Poly-L-ornithine solution has been used in the induction of differentiation.
Poly-L-ornithine has been used successfully for the attachement and study of neural cell differentiation and outgrowth. Recommended as a cell culture substratum when using 0.5 ml of a 0.1 mg/ml solution to coat 25 cm2.

Biochem/physiol Actions

Poly-L-ornithine helps to improve the penetration of substances into cells. It can induce the uptake of DNA.

Preparation Note

Prepared in cell culture grade water.

related product

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Ocular Neuroprotection (2003)
Ewoud B Compeer et al.
Nature communications, 9(1), 1597-1597 (2018-04-25)
Endocytosis of surface receptors and their polarized recycling back to the plasma membrane are central to many cellular processes, such as cell migration, cytokinesis, basolateral polarity of epithelial cells and T cell activation. Little is known about the mechanisms that
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Jafary H, et al.
Cell Biology International, 32(2), 278-286 (2008)

Articles

Discover L-ornithine, an important amino acid supplement, explore how L-ornithine is used in CHO and iPSC culture, and discover how poly-L-ornithine is used to enhance cell attachment.

Discover L-ornithine, an important amino acid supplement, explore how L-ornithine is used in CHO and iPSC culture, and discover how poly-L-ornithine is used to enhance cell attachment.

Discover L-ornithine, an important amino acid supplement, explore how L-ornithine is used in CHO and iPSC culture, and discover how poly-L-ornithine is used to enhance cell attachment.

Discover L-ornithine, an important amino acid supplement, explore how L-ornithine is used in CHO and iPSC culture, and discover how poly-L-ornithine is used to enhance cell attachment.

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Protocols

A stem cell culture protocol to generate 3D NSC models of Alzheimer’s disease using ReNcell human neural stem cell lines.

A stem cell culture protocol to generate 3D NSC models of Alzheimer’s disease using ReNcell human neural stem cell lines.

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