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Sigma-Aldrich

Atto 532

BioReagent, suitable for fluorescence, ≥90% (HPCE)

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About This Item

MDL number:
MFCD05865400
UNSPSC Code:
12352108
NACRES:
NA.25

product line

BioReagent

Assay

≥90% (HPCE)

manufacturer/tradename

ATTO-TEC GmbH

fluorescence

λex 533 nm; λem 560 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence

storage temp.

−20°C

General description

Atto 532 is a new label with high molecular absorption (115,000) and quantum yield (0.90) as well as sufficient Stoke′s shift between excitation and emission maximum. It is optimized for excitation with frequency doubled Nd:YAG-Laser, and is characterized by high photostability.

Application

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.
Atto 532 has been conjugated with the secondary antibodies for STED (stimulated emission depletion) microscopy and immunofluorescence studies.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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3D reconstruction of high-resolution STED microscope images.
Punge A et al.
Microscopy Research and Technique, 71, 644-644 (2008)
Uffe V Schneider et al.
BMC biotechnology, 10, 4-4 (2010-01-28)
Melting temperature of DNA structures can be determined on the LightCycler using quenching of FAM. This method is very suitable for pH independent melting point (Tm) determination performed at basic or neutral pH, as a high throughput alternative to UV
Andrea Armbrüster et al.
FEBS letters, 579(9), 1961-1967 (2005-03-29)
The ability of subunit C of eukaryotic V-ATPases to bind ADP and ATP is demonstrated by photoaffinity labeling and fluorescence correlation spectroscopy (FCS). Quantitation of the photoaffinity and the FCS data indicate that the ATP-analogues bind more weakly to subunit
STED microscopy reveals nanoparticle assemblies
Willig, K.I., et al.
New Journal of Physics, 8, 1-1 (2006)
Daniel Aquino et al.
Nature methods, 8(4), 353-359 (2011-03-15)
We demonstrate three-dimensional (3D) super-resolution imaging of stochastically switched fluorophores distributed across whole cells. By evaluating the higher moments of the diffraction spot provided by a 4Pi detection scheme, single markers can be simultaneously localized with <10 nm precision in
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