EZHTNFA-150K

Human TNF-α ELISA Kit

detects TNF-α in 50 μL serum, plasma or cell culture supernatent

• Synonym(s): Human TNF-A ELISA, TNF-A ELISA, TNF-Alpha, Tumour Necrosis Factor Alpha ELISA
• UNSPSC Code: 41116158
• NACRES: NA.77

Properties

• product name: Human TNF-α Conferma^® ELISA Kit, kit sufficient for 96 wells
• Quality Level: 200
• species reactivity: human
• packaging: kit of 1 × 96 wells
• parameter: 50 μL sample volume (4hr assay)
• assay range: linearity: 89-113%; (Serum, 101%); linearity: 98-110%; (Plasma, 103%); recovery range: 81-111%; (Plasma, 97%); recovery range: 85-105%; (Serum,95%); inter-assay cv: ≤7%; intra-assay cv: ≤3%; sensitivity: 1.65 pg/mL; standard curve range: 1.65-400 pg/mL
• technique(s): ELISA: suitable
• input: sample type cell culture supernatant; sample type serum; sample type plasma (K2 EDTA)
• application(s): research use
• detection method: colorimetric (450nm/590nm)
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

TNF-α is a core biomarker for the study of inflammation, considered to be a major contributing factor to numerous diseases and pathological processes. The Conferma^® ELISA for TNF-α uses highly characterized critical reagents assessed by LC-MS, RPLC-UV-MS and Surface Plasmon Resonance (SPR) to deliver ensure similarity and that the mAb / Standard relationship is maintained . Each assay is further anchored against a recombinant gold standard and a sample library to ensure strong finished kit performance lot to lot. Detailed information can be found in the Protocol & Assay Verification Report.

Research Category: Inflammation

Research Sub-Category:
Alzheimer′s Disease
Aging
Inflammation
Neurodegenerative Diseases
Metabolic Disease
Sepsis Viral Diseases
Cardiovascular Disease

Specificity

This assay was tested for cross reactivity with other human cytokines like IL-1a, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12, IL-13, IL-15, IL-17A, CCL3, CCL4, MCP-1, TNFα, GMCSF, VEGF. There is no cross-reactivity detected with any analyte.

Application

For the detection of TNF-α by ELISA in Human Serum & Plasma.

Features and Benefits

Capture ELISA assay. Highly Verified. Suitable for 37 samples in Duplicate, with a 6 point Standard Curve, 3 Quality Controls and a Blank.

Legal Information

CONFERMA is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Pictograms: GHS05,GHS07,GHS08
• Signal Word: Danger
• Hazard Statements: H290 - H302 + H312 + H332 - H317 - H318 - H373 - H412
• Precautionary Statements: P273 - P280 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338 - P314
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Skin Sens. 1 - STOT RE 2
• Target Organs: Respiratory Tract
• Storage Class Code: 8A - Combustible, corrosive hazardous materials
• WGK: WGK 3