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N6013

Sigma-Aldrich

Nutrient Mixture F-10 Ham

With sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Ham’s F-10

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About This Item

MDL number:
MFCD00217607
UNSPSC Code:
12352207
NACRES:
NA.75

product name

Nutrient Mixture F-10 Ham, With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

HEPES: no
phenol red: yes
L-glutamine: no
sodium pyruvate: 0.11 g/L
NaHCO3: yes

shipped in

ambient

storage temp.

2-8°C

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General description

F-10 Ham supports the growth of human diploid cells, white blood cells for chromosomal analysis, and primary explants of rat, rabbit, and chicken tissues. Ham′s Nutrient Mixtures are developed to support the clonal growth of several clones like Chinese hamster ovary (CHO) cells, clones of HeLa, and mouse L-cells.

Application

Nutrient Mixture F-10 Ham has been used:

  • to culture lamina cribrosa explants
  • in in vitro culture of lizard tail regenerate
  • for culturing of lizard embryo-fibroblasts

Reconstitution

Supplement with 0.146 g/L L-glutamine.

also commonly purchased with this product

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Description
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supplement

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Description
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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Elisabetta Polazzi et al.
Tissue & cell, 43(6), 350-358 (2011-08-30)
An in vitro system of isolated skin cells has been developed in order to address the understanding on the factors that control the shedding cycle and differentiation of lizard epidermis. The skin from the regenerating lizard tail has been separated
Navita N Lopez et al.
Experimental eye research, 197, 108103-108103 (2020-06-12)
The lamina cribrosa is the initial site of glaucomatous injury. Pathological changes to the lamina cribrosa include posterior displacement of the lamina cribrosa, loss of trophic support, and remodeling of the extracellular matrix. Optic nerve head (ONH) astrocytes and lamina
Roser Buscà et al.
BMC evolutionary biology, 15, 179-179 (2015-09-04)
The Ras/Raf/MEK/ERK signaling pathway is involved in essential cell processes and it is abnormally activated in ~30 % of cancers and cognitive disorders. Two ERK isoforms have been described, ERK1 and ERK2; ERK2 being regarded by many as essential due

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