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K3388

Sigma-Aldrich

Kappa Light Chains (Free) from human myeloma sera

purified immunoglobulin, ~1.0 mg/mL, ≥95% (SDS-PAGE), buffered aqueous solution

Synonym(s):

Free Kappa Light Chains, Kappa Light Chains

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

human

Quality Level

antibody form

purified immunoglobulin

Assay

≥95% (SDS-PAGE)

form

buffered aqueous solution

concentration

~1.0 mg/mL

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

κ Light Chains (free) is obtained from human IgG κ myeloma sera. Fractionation, ion-exchange, and affinity chromatography procedures are used for purification.

Application

Purified κ light chains may be used as an immunoglobulin calibrator, reference antigen, blocking agent or coating protein in a variety of immunoassays including ELISA, dot-blot immunobinding, Western immunoblotting, immunodiffusion, immunoelectrophoresis, hemagglutination, and cell-binding assays.

Physical form

Solution in 0.01 M phosphate and 0.15 M sodium chloride, pH 7.2, containing 1.0 M glycine and 0.1% sodium azide.

Preparation Note

Purified from pooled IgG kappa myeloma sera.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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