Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

G4410

Sigma-Aldrich

Griess reagent (modified)

Synonym(s):

Greiss, nitrite assay

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

EC Number:
215-981-2
UNSPSC Code:
12352200
NACRES:
NA.32

form

powder

usage

10 g

General description

Griess reagent is based on Griess reaction. The components include naphthylethylenediamine dihydrochloride suspended in water and sulphanilamide in phosphoric acid. This reagent reacts with nitrite in samples to form a purple azo product, absorbance of which is measured at 546 nm.

Application

Griess reagent (modified) has been used:
  • in the nitrite assay of primary spinal cord glial cells stimulated with supernatant of damaged sensory neuron (SDSN)
  • for measuring nitrite/nitrate oxidized from nitric oxide in stimulated chondrocytes
  • for measuring nitric oxide (NO) degradation in RAW264.7 macrophage cells
  • to measure the concentration of nitric oxide(NO) in the conditioned media from cultured macrophages (MΦs)
  • to determine the relative levels of NO released by BEAS-2B bronchial cells in the culture supernatants

Pictograms

Corrosion
GHS05

Signal Word

Danger

Hazard Statements

H314

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

WGK

WGK 2

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

The C-terminal sequence of several human serine proteases encodes host defense functions
Kasetty G, et al.
Journal of Innate Immunity, 3(5), 471-471 (2011)
A critical role of toll-like receptor 2 in nerve injury-induced spinal cord glial cell activation and pain hypersensitivity
Kim D, et al.
The Journal of Biological Chemistry, 282(20), 14975-14983 (2007)
Analysis of nitrate, nitrite, and [15N] nitrate in biological fluids
Green LC, et al.
Analytical biochemistry, 126(1), 131-138 (1982)
Effects of pleiotrophin, a heparin-binding growth factor, on human primary and immortalized chondrocytes
Pufe T, et al.
Osteoarthritis and Cartilage, 15(2), 155-162 (2007)
Praveen Papareddy et al.
The Journal of biological chemistry, 285(36), 28387-28398 (2010-07-02)
Tissue factor pathway inhibitor (TFPI) inhibits tissue factor-induced coagulation, but may, via its C terminus, also modulate cell surface, heparin, and lipopolysaccharide interactions as well as participate in growth inhibition. Here we show that C-terminal TFPI peptide sequences are antimicrobial
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service