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biological source
Mouse x Mouse Hybridoma
ImmunoDon
BALB/c mouse spleen,female
Myeloma
NS-1
Reactivity
Anti-CD36, human
technique(s)
cell culture | mammalian: suitable
isotype
IgM
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse x Mouse hybridoma
Cell Line Description
The hybridoma line 8C9 was produced by immunizing a female BALB/c mouse i.p. with tissue from a human malignant fibrous histiocytoma once a week for 6 weeks. Mouse spleen cells were fused with myeloma NS-1 cells using PEG and HAT for selection . The 8C9 monoclonal antibody detects a unique epitope on the leukocyte differentiation antigen CD36 (platelet glycoprotein IV or III6) expressed by normal and neoplastic endothelial cells. Immunoreactivity with 8C9 was detected on capillary endothelial cells, adipocytes, monocytes, platelets and the human monocytoid cell line U-937 (Sigma Catalogue number. 85011440). The antibody did not react with erythroid cells, T cells, B cells or HUVECs (human umbilical vein endothelial cells). Immunoblot analysis showed that 8C9 bound to a 97-kDa membrane protein expressed by U-937 cells treated with phorbol ester. Comparative immunohistochemical studies using 8C9 and the monoclonal antibody OKM demonstrated epitopic heterogeneity of the CD36 antigen by normal and neoplastic endothelial cells.
Immunogen
Human malignant fibrous histiocytoma
Application
Study of endothelial cells, CD36 expression analysis
Culture Medium
RPMI 1640 + 2mM glutamine + 10% FBS
Subculture Routine
Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO2; 37°C
Other Notes
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Certificates of Analysis (COA)
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