A2058
91100402, human skin (lymph node metastasis), Epithelial
Synonym(s):
A2058 Cell Line
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About This Item
UNSPSC Code:
41106514
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product name
A2058, 91100402
biological source
human skin (lymph node metastasis)
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Nerve growth factor (NGF), Laminin
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
metastasis
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human caucasian metastatic melanoma
Cell Line Description
This cell line has been isolated from a lymph node metastasis from a 43 year old male patient with malignant melanoma. Due to their highly invasive properties, the cells can be used as a source of cellular invasion associated proteins e.g. collagen type IV collagenase, tissue inhibitor of metalloproteinase-2 and autocrine motility factor.
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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