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Key Documents

06-661

Sigma-Aldrich

Anti-Calreticulin Antibody

Upstate®, from rabbit

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

pig, human, mouse, Xenopus, bovine, rat

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CALR(811)

Specificity

calreticulin; does not cross react with other endoplasmic reticulum proteins

Immunogen

peptide (QAKDEL) corresponding to the C-terminus (amino acids 412-417) of human calreticulin

Application

Anti-Calreticulin Antibody is an antibody against Calreticulin for use in IC, IH & WB.
Research Sub Category
PI3K, Akt, & mTOR Signaling

Enzymes & Biochemistry

Quality

routinely evaluated by immunoblot on RIPA lysates from murine 3T3/NIH fibroblasts

Target description

60kDa

Linkage

Replaces: MABT145

Physical form

0.1M Tris-glycine, pH 7.0, 0.15M NaCl and 0.05% sodium azide.
Format: Purified

Analysis Note

Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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