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  • Experimentally induced psoriatic lesions associate with rapid but transient decrease in interleukin-33 immunostaining in epidermis.

Experimentally induced psoriatic lesions associate with rapid but transient decrease in interleukin-33 immunostaining in epidermis.

Acta dermato-venereologica (2014-11-27)
Mireille-Maria Suttle, Mattias Enoksson, Anna Zoltowska, Madhumita Chatterjee, Gunnar Nilsson, Ilkka T Harvima
ABSTRACT

A slight epidermal damage can induce the Köbner reaction in psoriasis, and the “alarmin”, interleukin-33 (IL-33), may be involved in this process. Therefore, the uninvolved psoriatic skin was tape-stripped, and skin biopsies were collected at 0 day, 2 h and 3 days or at 0 day, 1 day and 7 days for immunohistochemistry. Eight patients out of 18 with the positive Köbner reaction showed a decrease in epidermal thickness and revealed transient reduction in epidermal nuclear immunostaining of IL-33 in 2-h, 1-day, 3-day biopsies compared to the 10 Köbner-negative patients. In keratinocyte cultures, the full-length 32-kDa IL-33 was detected after damaging the cells with freeze-thawing. Interestingly, a very low concentration of rh-IL-33 (0.001–0.01 ng/ml) significantly stimulated (3)H-thymidine uptake by human LAD2 mast cells, but not by psoriatic peripheral blood mononuclear cells. The results show that epidermal IL-33 associates with positive Köbner response, and only a small amount of the IL-33 apparently released may induce proliferation in dermal mast cells.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
N-Chlorosuccinimide, 98%
Sigma-Aldrich
Stem Cell Factor human, SCF, recombinant, expressed in E. coli, powder, suitable for cell culture
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Stem Cell Factor human, SCF, recombinant, expressed in HEK 293 cells, suitable for cell culture
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SCF human, Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture