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MAK122

Sigma-Aldrich

Phospholipid Assay Kit

sufficient for 100 colorimetric or fluorometric tests

Synonym(s):

Phospholipid Quantification Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 colorimetric or fluorometric tests

application(s)

cosmetics
food and beverages
pharmaceutical

detection method

colorimetric
fluorometric

shipped in

dry ice

storage temp.

−20°C

General description

Phospholipids are a class of lipids which constitute a major component of cell membranes and play important roles in signal transduction. Most phospholipids contain one diglyceride, a phosphate group, and one choline.

In this assay, phospholipids (such as lecithin, lysolecithin and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H2O2-specific dye. This results in a colorimetric (570 nm)/ fluorometric (λex = 530/λem = 585 nm) product directly proportional to the phospholipid concentration in the sample. The range of linear detection is 3 - 200 μM for colorimetric assays and 0.6 - 20 μM for fluorometric assays.

Application

Phospholipid assay kit has been used in biochemical analysis and phospholipid quantitation.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the detection of phospholipids in biological samples

Principle

In this assay, phospholipids (such as lecithin, lysolecithin and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H2O2-specific dye. This results in a colorimetric (570 nm)/ fluorometric (λex = 530/λem = 585 nm) product directly proportional to the phospholipid concentration in the sample. The range of linear detection is 3 - 200 μM for colorimetric assays and 0.6 - 20 μM for fluorometric assays.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Aquatic Chronic 3 - Resp. Sens. 1

Storage Class Code

10 - Combustible liquids


Certificates of Analysis (COA)

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