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Key Documents

L7395

Sigma-Aldrich

Lipoxidase from Glycine max (soybean)

Type I-B, lyophilized powder, ≥50,000 units/mg solid

Synonym(s):

15-lipoxygenase, LOX A, Linoleate:oxygen oxidoreductase, Lipoxygenase

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About This Item

CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

type

Type I-B

form

lyophilized powder

specific activity

≥50,000 units/mg solid

mol wt

94 kDa

shipped in

wet ice

storage temp.

−20°C

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General description

Lipoxidases (LOXs), or lipoxygenases, are nonheme iron proteins. Lipoxygenases are widely present in plants, some microorganisms, and animals. They are categorized as LOX-1, LOX-2, and LOX-3 with respect to pH and optimum temperature, substrate specificity, and level of inhibition by different agents.

Application

Lipoxidase from Glycine max (soybean) has been used:
  • to study the effects of 2-(α-D-glucopyranosyl)methyl-2,5,7,8-tetramethylchroman-6-ol(TMG) on oxidation of lipoproteins
  • as a representative enzyme for lipoxygenases in microplate assay platform to screen for enzymes degrading wood extractives
  • as a positive control in lipoxygenase inhibitory activity assay

The soybean enzyme will use arachidonic acid as a substrate, with ~ 15% of the activity indicated using linoleic acid as the substrate; the product of arachidonic acid oxidation is 12- or 15-hydroperoxyarachidonic acid (12-HPETE or 15-HPETE).

Biochem/physiol Actions

Lipoxidases are deactivated by heat treatment near 100°C for 5 to 10 minutes. Studies show that gamma irradiation treatment decreases the specific activity of soybean lipoxygenases. These enzymes are used in studies related to asthma and allergies. Lipoxidases are applicable for industrial bio-transformations such as in the production of aroma fine chemicals.
Catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure.

Unit Definition

One unit will cause an increase in A234 of 0.001 per min at pH 9.0 at 25 °C when linoleic acid is the substrate in 3.0 ml volume (1 cm light path). One A234 unit is equivalent to the oxidation of 0.12 μmole of linoleic acid.

Physical form

Contains stabilizer and NaCl

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Chemical composition and lipoxygenase activity in soybeans (Glycine max L. Merr.) submitted to gamma irradiation
de Barros EA, et al.
Radiation Physics and Chemistry, 98, 29-32 (2014)
Ewelina Zielińska et al.
Nutrients, 9(9) (2017-09-05)
This study investigated the effect of heat treatment of edible insects on antioxidant and anti-inflammatory activities of peptides obtained by in vitro gastrointestinal digestion and absorption process thereof. The antioxidant potential of edible insect hydrolysates was determined as free radical-scavenging
Xiao-Hui Ma et al.
Signal transduction and targeted therapy, 7(1), 288-288 (2022-08-16)
Myocardial ischemia/reperfusion (I/R) injury is a classic type of cardiovascular disease characterized by injury to cardiomyocytes leading to various forms of cell death. It is believed that irreversible myocardial damage resulted from I/R occurs due to oxidative stress evoked during
Microplate enzyme assay for screening lipoxygenases to degrade wood extractives
Nguyen D, et al.
Biocatalysis and Biotransformation, 25(2-4), 202-210 (2007)
Sereyvath Yoeun et al.
Frontiers in chemistry, 6, 500-500 (2018-11-15)
Allene oxide cyclase (AOC) is a key enzyme in the jasmonic acid (JA) biosynthetic pathway in plants, during which it catalyzes stereospecific conversion of 12,13(S)-epoxy-9(Z),11,15(Z)-octadecatrienoic acid (12,13-EOT) to cis(+)-12-oxophytodienoic acid. Here, rice allene oxide cyclase (OsAOC) was localized to the

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