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Key Documents

SM-2004

Sigma-Aldrich

Low Trypsin-High EDTA

PBS Based, 0.025% Trypsin & 0.75mM EDTA (1X), without Ca 2+ & Mg 2+

Synonym(s):

Low Trypsin-High EDTA Solution, PBS-Based Trypsin Solution, Trypsin-EDTA PBS

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About This Item

UNSPSC Code:
12352204
eCl@ss:
32160801
NACRES:
NA.75

product name

Low Trypsin-High EDTA, PBS Based, 0.025% Trypsin and 0.75mM EDTA (1X), without Ca2+ and Mg2+, The Low Trypsin-High EDTA, PBS Based, 0.025% Trypsin & 0.75mM EDTA (1X), without Ca 2+ & Mg 2+ is available in a 100 mL format.

Quality Level

form

liquid

manufacturer/tradename

Specialty Media

input

sample type: human embryonic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type epithelial cells
sample type hematopoietic stem cell(s)
sample type mesenchymal stem cell(s)
sample type neural stem cell(s)
sample type pancreatic stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type cardiac stem cell(s)

Application

Low Trypsin-High EDTA, PBS Based, 0.025% Trypsin and 0.75mM EDTA (1X), without Ca2+ and Mg2+ has been used:
  • as an incubating medium for single-cell preparation of human islets
  • in the preparation of mouse embryonic fibroblast cells stock for conventional gene targeting
  • to trypsinize human umbilical vein endothelial cells (HUVECs) for coating the beads with HUVECs

Storage and Stability

Store at -20°C for 18 months.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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