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324681

Sigma-Aldrich

Elastase, Human Neutrophil

Elastase, Human Neutrophil, CAS 9004-06-2, is a serine protease that degrades elastin, collagen, and proteoglycans

Synonym(s):

Elastase, Human Neutrophil

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About This Item

CAS Number:
Enzyme Commission number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.77

Quality Level

form

lyophilized solid

specific activity

≥20 units/mg protein

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

shipped in

ambient

storage temp.

−20°C

General description

Elastase, Human Neutrophil, CAS 9004-06-2, is a serine protease that degrades elastin, collagen, and proteoglycans
Native elastase from human neutrophil. A serine protease that degrades elastin, collagen, and proteoglycans. Its overexpression has been implicated in the development of pulmonary emphysema, rheumatoid arthritis, and cystic fibrosis.
Native elastase from human neutrophil. Degrades collagen, elastin, and proteoglycans. Elastase has been implicated in the development of pulmonary emphysema and rheumatoid arthritis.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of MeO-Suc-Ala-Ala-Pro-Val-pNA (Cat. No. 454454) per min at 25°C, pH 8.0.

Physical form

Salt-free.

Preparation Note

Prepared from blood that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Acidic stock solutions are stable for up to 1 month at 4°C or for up to 1 year at -20°C.
Reconstitute in 50 mM sodium acetate, pH 5.5, with 200 mM NaCl or in other aqueous buffers. Material is most active near a neutral pH, but to maintain stability upon reconstitution, use in a slightly acidic buffer (pH 5.5) to avoid autolysis.

Analysis Note

Major band by SDS-PAGE

Other Notes

Siedle, B., et al. 2002. Biorg. Med. Chem.10, 2855.
Junger, W.G., et al. 1992. Biol. Chem. Hoppe-Seyler 373, 691.
Selak, M.A. 1992. Thromb. Haemost.68, 570.
Baugh, R.J., and Travis, J. 1976. Biochemistry15, 836.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Health hazardExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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