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HPLC Analysis of Lysozyme on SRT® SEC-300, 30 cm x 7.8 mm I.D., 5 μm particles

HPLC Analysis of Lysozyme on SRT® SEC-300, 30 cm x 7.8 mm I.D., 5 μm particles application for HPLC

Materials

standard

Product No.
Description
Pricing

L4631

Lysozyme from chicken egg white

For use as a marker in SDS-PAGE

L6876

Lysozyme from chicken egg white

powder or granules, ≥90 %, ≥39,000 units/mg protein

CONDITIONS

column

SRT SEC-300, 30 cm x 7.8 mm I.D., 5 μm particles (Z777051)

mobile phase

50 mM phosphate buffered saline, 200 mM Na2SO4, pH 7.0

flow rate

1 mL/min

column temp.

ambient

detector

UV, 214 nm

injection

5 μL

sample

1 mg/mL in 50 mM phosphate buffered saline

Description

Analysis Note

The positively-charged protein, lyzoyme (pI=11), exhibits excellent peak shape and recovery on SRT SEC-300 columns due to its highly-inert surface. Low protein recovery is usually attributed to 1) hydrophobic interactions with the stationary phase and 2) electrostatic interactions with the silica suface. SRT SEC-300 surface is hydrophilic and densely bonded to silica, therefore providing high mass recovery and good peak shape.

Legal Information

SRT is a registered trademark of Sepax Technologies

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