HPLC Analysis of Exenatide Acetate on Proteomix® SCX-NP5
Materials
analytical column
Product No.
Description
Pricing
Proteomix® SCX-NP5 Strong Cation Exchange Column
L × I.D. 25 cm × 4.6 mm, 5 μm particle size, stainless steelCONDITIONS
column
Proteomix SCX NP5, 25 cm x 4.6mm I.D., 5 μm particles (Z777161)
mobile phase
(A) 10 mM monosodium phosphate:acetonitrile (56:44, v/v), adjusted to pH to 2.0 with phosphoric acid; ((B) 0.5 M NaCl/10 mM monosodium phosphate:acetonitrile (56:44, v/v), adjusted to pH to 2.0 with phosphoric acid)
gradient
0 to 65% B in 35 min; held for 5 min; to 0% B in 10 min
flow rate
1.0 mL/min
pressure
1653 psi (114 bar)
column temp.
40 °C
detector
UV, 235 nm
injection
20 μL
sample
0.325 mg/mL exenatide acetate in mobile phase A
Description
Legal Information
Proteomix is a registered trademark of Sepax Technologies