HPLC Analysis of Bupivacaine Enantiomers on Astec^® CHIROBIOTIC^® V2, Acetate Mobile Phase

column

CHIROBIOTIC V2, 25 cm x 4.6 mm I.D., 5 μm particles (15024AST)

mobile phase

[A] methanol; [B] 20 mM ammonium acetate, pH 4.1; (80:20, A:B)

flow rate

1 mL/min

column temp.

25 °C

detector

UV, 205 nm

injection

10 μL

sample

1 mg/mL in methanol