HPLC Analysis of 2-Bromo-3-Methylbutyric Acid Enantiomers on Astec^® CHIROBIOTIC^® R

column

CHIROBIOTIC R, 25 cm x 4.6 mm I.D., 5 μm particles (13024AST)

mobile phase

[A] 20 mM ammonium acetate; [B] methanol; (15:85, A:B)

(alternate mobile phase of 0.1% ammonium acetate in methanol gives 3.5 and 4.3 min retention)

flow rate

1 mL/min

column temp.

25 °C

detector

UV, 254 nm

injection

5 μL

sample

2 mg/mL in methanol