GC Analysis of Bacterial Acid Methyl Esters (BAMEs) on SUPELCOWAX 10

column

SUPELCOWAX 10, 30 m x 0.32 mm I.D., 0.25 μm (24082)

oven

50°C (1 min), 5 °C/min to 225 °C (5 min)

inj. temp.

250 °C

detector

FID, 250 °C

carrier gas

helium, 25 cm/sec (set at 200 °C)

injection

1 μL 100:1 split

liner

4 mm I.D., cup style

sample

bacterial methyl ester standards in methyl caproate, 10 mg/mL (47080-U)