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  • Utilization of microparticles in next-generation assays for microflow cytometers.

Utilization of microparticles in next-generation assays for microflow cytometers.

Analytical and bioanalytical chemistry (2010-06-08)
Jason S Kim, Frances S Ligler
ABSTRACT

Micron-sized particles have primarily been used in microfabricated flow cytometers for calibration purposes and proof-of-concept experiments. With increasing frequency, microparticles are serving as a platform for assays measured in these small analytical devices. Light scattering has been used to measure the agglomeration of antibody-coated particles in the presence of an antigen. Impedance detection is another technology being integrated into microflow cytometers for microparticle-based assays. Fluorescence is the most popular detection method in flow cytometry, enabling highly sensitive multiplexed assays. Finally, magnetic particles have also been used to measure antigen levels using a magnetophoretic micro-device. We review the progress of microparticle-based assays in microflow cytometry in terms of the advantages and limitations of each approach.

MATERIALS
Product Number
Brand
Product Description

Supelco
Micro particles based on silicon dioxide, size: 0.5 μm
Supelco
Micro particles based on silicon dioxide, size: 1.0 μm