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  • Targeted analysis of ceramides and cerebrosides in yellow lupin seeds by reversed-phase liquid chromatography coupled to electrospray ionization and multistage mass spectrometry.

Targeted analysis of ceramides and cerebrosides in yellow lupin seeds by reversed-phase liquid chromatography coupled to electrospray ionization and multistage mass spectrometry.

Food chemistry (2020-04-29)
Mariachiara Bianco, Cosima Damiana Calvano, Ilario Losito, Francesco Palmisano, Tommaso R I Cataldi
ABSTRACT

Ceramides (Cer) and cerebrosides are important sphingolipids (SL) involved in many biological processes. Herein, the SL content of yellow lupin seeds (Lupinus luteus) was determined by liquid-liquid extraction, mild alkaline hydrolysis (1 h at 37 °C) and reversed-phase liquid chromatography with negative electrospray ionization coupled to either an orbital-trap (Fourier-transformed, FT) or linear ion-trap (LIT) mass spectrometry (RPLC-ESI/MS). The chemical identity of SL including the sugar residues and sphingoid backbone (SB) was inferred by collision-induced dissociation multiple-stage mass spectrometry (MSn, n = 2,3). Up to 52 Cer and 47 cerebrosides were successfully recognized and quantified in sample extracts of L. luteus seeds also counting isobaric species. As reported for other vegetables, a hydroxylated SB was observed whereby the N-acyl chains showed a high occurrence of very-long-chain moieties; phytosphingosine and 4-hydroxy-8-sphingenine were the predominant SB paired mainly with oleic acid (C18:1), hydroxylated behenic acid (C22:0;1) and hydroxylated lignoceric acid (C24:0;1).

MATERIALS
Product Number
Brand
Product Description

Avanti
C17 Ceramide (d18:1/17:0), Avanti Research - A Croda Brand
Avanti
C12 Mono-Sulfo Galactosyl(β) Ceramide (d18:1/12:0), Avanti Research - A Croda Brand 860573P, powder
Avanti
18:0 Lyso PI, 1-stearoyl-2-hydroxy-sn-glycero-3-phosphoinositol (ammonium salt), powder