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  • Binding of barrier to autointegration factor (BAF) to histone H3 and selected linker histones including H1.1.

Binding of barrier to autointegration factor (BAF) to histone H3 and selected linker histones including H1.1.

The Journal of biological chemistry (2005-10-06)
Rocío Montes de Oca, Kenneth K Lee, Katherine L Wilson
ABSTRACT

Barrier to autointegration factor (BAF) is an essential conserved double-stranded DNA-binding protein in metazoans. BAF binds directly to LEM domain nuclear proteins (e.g. LAP2, Emerin, and MAN1), lamin A, homeodomain transcription factors, and human immunodeficiency virus type 1-encoded proteins. BAF influences higher order chromatin structure and is required to assemble nuclei. BAF also facilitates retroviral preintegration complex insertion into target DNA in vitro, through unknown mechanisms. We report that BAF binds directly and selectively to linker histone H1.1 (among three subtypes tested) and core histone H3 with affinities of approximately 700 nm and approximately 100-200 nm, respectively, in vitro and in vivo. Mutations at the bottom and top surfaces of the BAF dimer disrupted or enhanced, respectively, this binding and affected H1 and H3 similarly. Biochemical studies showed that C-terminal residues 108-215 of histone H1.1 and the N-terminal tail plus helix alphaN in the core of histone H3.1 were each necessary and sufficient to bind BAF. Based on its interactions with histones and DNA, we propose BAF might bind nucleosomes in vivo.

MATERIALS
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Sigma-Aldrich
Histone H1 Protein, 20 mg, Purified bovine Histone H1 protein for use as a substrate in histone modification assays (HAT, HDAC, DNMT) and chromatin assembly studies.