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Key Documents

G006118

HPLC Analysis of a Protein Mixture on Zenix® SEC-150 versus Zenix® SEC-300, Effect of Pore Size on Resolution

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Zenix SEC-150, 30 cm x 7.8 mm I.D., 3 μm, 150 Å particles (Z777018)
column: Zenix SEC-300, 30 cm x 7.8 mm I.D., 3 μm, 300 Å particles (Z777033)
mobile phase: 150 mM sodium phosphate, pH 7
flow rate: 1 mL/min
column temp.: ambient
detector: UV, 214 nm
injection: 10 μL
sample: protein mixture

suitability

application for HPLC

application(s)

clinical
pharmaceutical (small molecule)

Legal Information

Zenix is a registered trademark of Sepax Technologies

Analyte

Description
    Thyroglobulin from bovine thyroid, powder, ≥90% (agarose gel electrophoresis)
    γ-Globulins from human blood, ≥99% (electrophoresis)
    Ovalbumin (257-264) chicken, ≥97% (HPLC)
    Ribonuclease A from bovine pancreas, for molecular biology, ≥70 Kunitz units/mg protein, lyophilized
    4-Aminobenzoic acid, analytical standard

analytical column

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standard

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