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Key Documents

SRP6147

Sigma-Aldrich

Ubiquitin Activating Enzyme E1 human

≥95% (SDS-PAGE)

Synonym(s):

AMCX1, POC20, UBA1, UBE1, Ubiquitin E1

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

Assay

≥95% (SDS-PAGE)

form

liquid

potency

>3 nM

mol wt

118

packaging

pkg of 50 μg

NCBI accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... UBA1(7317)

General description

Research area: Cell Signaling

Ubiquitin activating enzyme 1 (UBA1) or UBE1, is an ubiquitin-activating enzyme. The gene encoding it is localized on human chromosome Xp11.23.

Application

Ubiquitin Activating Enzyme E1 human has been used in in vitro and in vivo ubiquitination assays.

Biochem/physiol Actions

Ubiquitin activating enzyme 1 (UBA1) has a role in cellular protein homeostasis. It is the first enzyme utilized in the pathway to conjugate ubiquitin onto target substrates through an ubiquitin-protein isopeptide bond. The C-terminal glycine of an ubiquitin (G76) is initially adenylated in an ATP dependent manner activating it so it can be transferred to the active site cysteine of UBE1 forming an ubiquitin-UBE1 thiolester. With the help of the other enzymes in the ubiquitin conjugation pathway (E2/E3 enzymes) the ubiquitin is transferred to a specific lysine on a target substrate. The protein has been associated with spinal muscular atrophy (SMA) and Huntington′s disease (HD).

Physical form

In 50 mM Tris (pH 8.0), 100 mM NaCl and 10% glycerol.

Preparation Note

Centrifuge the vial prior to opening.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Structure of a ubiquitin E1-E2 complex: insights to E1-E2 thioester transfer.
Olsen SK and Lima CD
Molecular Cell, 49(5), 884-896 (2013)
UBA1: At the Crossroads of Ubiquitin Homeostasis and Neurodegeneration.
Groen EJ and Gillingwater TH
Trends in Molecular Medicine, 21(10), 622-632 (2015)
E2 enzymes: more than just middle men.
Stewart MD
Cell Research, 26(4), 423-440 (2016)
Jung Hwa Lim et al.
Cellular and molecular life sciences : CMLS, 76(13), 2647-2662 (2019-03-25)
Hepatitis B virus (HBV) X protein (HBx) is associated with hepatocarcinogenesis. E2-EPF ubiquitin carrier protein (UCP) catalyzes ubiquitination of itself and von Hippel-Lindau protein (pVHL) for degradation and associates with tumor growth and metastasis. However, it remains unknown whether HBx
UBE1 expression in extranodal NK/T cell lymphoma, nasal type.
Z L Su et al.
Leukemia & lymphoma, 49(9), 1821-1822 (2008-07-29)

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