SAB4200805

Anti-β-Galactosidase antibody, Mouse monoclonal

clone GAL-13, purified from hybridoma cell culture

• Synonym(s): Anti-Beta-gal, Anti-Beta-galactosidase, Anti-Lactase
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: GAL-13, monoclonal
• description: Research area: Microbiome
• form: buffered aqueous solution
• species reactivity: E. coli
• packaging: antibody small pack of 25 μL
• concentration: ~1.0 mg/mL
• technique(s): immunofluorescence: suitable; immunohistochemistry: suitable; indirect ELISA: 0.5-1 μg/mL using anti-Mouse antibody for coating, primary Anti-Beta-GAL antibody followed by addition of β-d-galactosidase enzyme from E. coli and 2-Nitrophenyl β-D-galactopyranoside as substrate.
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: Escherichia coli K12 ... Lacz(945006)

Description

General description

Anti-nitric oxide synthase, inducible antibody, mouse monoclonal (mouse IgG1 isotype) is derived from the NOS-IN hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse immunized with a synthetic peptide corresponding to the C terminal region of nitric oxide synthase (NOS) of mouse macrophage origin (iNOS, also termed macNOS), conjugated to KLH

Beta galactosidases have been obtained from microorganisms such as fungi, bacteria and yeasts; plants, animals cells and from recombinant sources. This enzyme has two main applications; the removal of lactose from milk products for lactose intolerant people and the production of galactosylated products.Monoclonal Anti-Beta-Galactosidase recognizes soluble Beta-d-galactosidase and retains the enzymatic activity. The antibody reacts with the native enzyme and does not recognize denatured or reduced Beta-galactosidase. The Anti-Beta-Galactosidase antibody maybe useful tool for immunoenzymatic staining of blood and bone marrow smears or tissue sections. In addition, this product may be used as a primary antibody for the detection and purification of recombinant fusion hybrid proteins, which contain Beta-galactosidase (lacZ) in the cloning vector.

Immunogen

Beta-D-Galactosidase purified from E. coli

Application

Anti-Nitric Oxide Synthase, Inducible antibody, Mouse monoclonal has been used in: immunoblotting immunofluorescence fluorescence-activated cell sorting 3 (FACS3) immunohistochemistry

The antibody is recommended to use in various immunological techniques, including ELISA, Immunofluorescence and immunohistology.

Biochem/physiol Actions

NO is a messenger molecule mediating diverse functions including vasodilatation, neurotransmission, antimicrobial and anti-tumor activities. It is implicated as a pathogenic mediator in a variety of conditions, including central nervous system (CNS) disease states, such as the animal model of multiple sclerosis (MS) and experimental allergic encephalomyelitis.

Physical form

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

Other Notes

This product is for R&D use only, not for drug, household, or other uses.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable