MAK526
Diamine Oxidase Assay Kit
sufficient for 100 fluorometric tests
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Recommended Products
Quality Level
detection method
fluorometric
relevant disease(s)
cancer; immunological diseases; gastrointestinal diseases
shipped in
wet ice
storage temp.
−20°C
General description
Diamine oxidase (DAO) also known as histaminase or amine oxidase (copper containing), is an enzyme involved in the metabolism, oxidation, and inactivation of histamine in animals. Highest levels are observed in the digestive tract and placenta. An imbalance between histamine intake and the capacity for histamine degradation can lead to histamine intolerance (HIT). Measuring DAO activity in serum can be useful in diagnosing HIT.
Features and Benefits
- Linear detection range 0.5 to 6 U/L .
- Compatible with high-throughput handling systems.
Suitability
The kit is suitable for diamine oxidase activity in serum or plasma samples.
Principle
The Diamine Oxidase Assay Kit is based on the oxidation of putrescine to pyrroline plus NH3 and H2O2. The generated H2O2 is then used by HRP to oxidize a dye making it fluorescent. The increase in fluorescence at λEx = 530 nm/λEm = 585 nm is directly proportional to the enzyme activity.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Certificates of Analysis (COA)
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