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M7633

4-Methylumbelliferyl α-D-galactopyranoside

≥98% (TLC)

Synonym(s):

MU-alpha-GAL

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About This Item

Empirical Formula (Hill Notation):
C16H18O8
CAS Number:
38597-12-5
Molecular Weight:
338.31
UNSPSC Code:
12352204
NACRES:
NA.32
PubChem Substance ID:
24897170
EC Number:
254-031-1
Beilstein/REAXYS Number:
94674
MDL number:
MFCD00063278
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Quality Level

200

assay

≥98% (TLC)

form

powder

solubility

water: 50 mg/mL, clear, colorless to very faintly yellow

fluorescence

λex 317 nm; λem 374 nm (pH 9.1), λex 360 nm; λem 449 nm (Reaction product)

storage temp.

−20°C

SMILES string

CC1=CC(=O)Oc2cc(O[C@H]3O[C@H](CO)[C@H](O)[C@H](O)[C@H]3O)ccc12

InChI

1S/C16H18O8/c1-7-4-12(18)23-10-5-8(2-3-9(7)10)22-16-15(21)14(20)13(19)11(6-17)24-16/h2-5,11,13-17,19-21H,6H2,1H3/t11-,13+,14+,15-,16+/m1/s1

InChI key

YUDPTGPSBJVHCN-CHUNWDLHSA-N

Application

4-Methylumbelliferyl α-D-galactopyranoside has been used as an artificial substrate to assay α-galactosidase-A activity.[1][2][3]

Biochem/physiol Actions

4-Methylumbelliferyl α-D-galactopyranoside is a fluorogenic substrate for α-galactosidase-A.[4] The enzyme converts the substrate to form a blue fluorescent product, methylumbelliferyl.[3][4] The fluorescence is measured spectrophotometrically.[4]

Preparation Note

4-Methylumbelliferyl α-D-galactopyranoside is soluble in water (50 mg/ml), yielding a clear, colorless to faint yellow solution with the application of heat.

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This Item
M9766M852769591
4-Methylumbelliferyl α-D-galactopyranoside ≥98% (TLC)

Sigma-Aldrich

M7633

4-Methylumbelliferyl α-D-galactopyranoside

4-Methylumbelliferyl α-D-glucopyranoside α-glucosidase substrate

Sigma-Aldrich

M9766

4-Methylumbelliferyl α-D-glucopyranoside

4-Methylumbelliferyl α-L-fucopyranoside ≥97% (TLC)

Sigma-Aldrich

M8527

4-Methylumbelliferyl α-L-fucopyranoside

4-Methylumbelliferyl α-D-glucopyranoside ≥98% (TLC)

Sigma-Aldrich

69591

4-Methylumbelliferyl α-D-glucopyranoside

assay

≥98% (TLC)

assay

≥99% (TLC)

assay

≥97% (TLC)

assay

≥98% (TLC)

solubility

water: 50 mg/mL, clear, colorless to very faintly yellow

solubility

pyridine: 50 mg/mL, clear, colorless to faintly yellow

solubility

DMF: 100 mg/mL, clear, colorless to faintly yellow

solubility

DMSO: 50 mg/mL, clear, colorless to faintly yellow

Quality Level

200

Quality Level

300

Quality Level

200

Quality Level

200

form

powder

form

powder

form

powder

form

powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

fluorescence

λex 317 nm; λem 374 nm (pH 9.1), λex 360 nm; λem 449 nm (Reaction product)

fluorescence

-

fluorescence

λex 360 nm; λem 449 nm (Reaction product)

fluorescence

λex 316 nm; λem 375 nm (Reaction product), λex 317 nm; λem 374 nm (pH9.0), λex 360 nm; λem 449 nm, λex 365 nm; λem 445 nm in 0.1 M Tris pH 8.0 (α-glucosidase)

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000533359
0000533360
0000533359
0000533360
0000494410

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Nathaniel G Hentz et al.
Journal of laboratory automation, 19(2), 153-162 (2013-09-14)
This study illustrates how optimization of both liquid-handling accuracy and precision is critical to assay performance. The study was designed to examine (1) liquid-handling performance and (2) the effect of liquid-handling variability on two types of in vitro biochemical assays
Eugènia Ruiz-Cánovas et al.
Colloids and surfaces. B, Biointerfaces, 208, 112123-112123 (2021-09-28)
The capability of HeLa cells to internalize large spherical microparticles has been evaluated by using inorganic, magnetic microparticles of 1 and 2.8 µm of diameter. In both absence but especially under the action of a magnet, both types of particles were
Zhen-Dan Shi et al.
Analytical and bioanalytical chemistry, 394(7), 1903-1909 (2009-06-13)
Alpha-galactosidase A hydrolyzes the terminal alpha-galactosyl moieties from glycolipids and glycoproteins in lysosomes. Mutations in alpha-galactosidase cause lysosomal accumulation of the glycosphingolipid, globotriaosylceramide, which leads to Fabry disease. Small-molecule chaperones that bind to mutant enzyme proteins and correct their misfolding
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Global Trade Item Number

SKUGTIN
M7633-250MG04061835557738
M7633-100MG04061835546176
M7633-25MG04061835557745

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