L023050

DCA Deblock

configured for (ÄKTA^® and OligoPilot^®)

• UNSPSC Code: 41116004

Properties

• description: with Toluene
• type: for DNA synthesis; for DNA synthesis
• storage condition: dry at room temperature
• concentration: 42.2-51.6 g/L (DCA content, titration)
• impurities: ≤200 ppm water content (Karl Fischer)
• color: clear
• compatibility: configured for (ÄKTA^® and OligoPilot^®)

Description

General description

Deblock solution containing dichloroacetic acid (DCA) in toluene removes the dimethoxytrityl (DMT) protecting group from the 5′hydroxyl moiety of nucleotides already incorporated into the growing nucleic acid, prior to the addition of the next phosphoramidite. This solution contains toluene / Dichloroactic Acid (97/3 v/v).

Application

DCA Deblock solution facilitates removal of the DMT that allows the unprotected 5′hydroxyl moiety to react with a new phosphoramidite in a subsequent extension reaction.

Preparation Note

Toluene / Dichloroacetic acid 97/3 (v/v)

Legal Information

OligoPilot is a registered trademark of Cytiva

ÄKTA is a registered trademark of Cytiva

Safety Information

• Pictograms: GHS02,GHS08,GHS05,GHS07
• Signal Word: Danger
• Hazard Statements: H225,H304,H315,H318,H336,H351,H360FD,H362,H373,H412
• Precautionary Statements: P210 - P263 - P280 - P301 + P310 - P305 + P351 + P338 - P331
• Hazard Classifications: Aquatic Chronic 3 - Asp. Tox. 1 - Carc. 2 - Eye Dam. 1 - Flam. Liq. 2 - Lact. - Repr. 1B - Skin Irrit. 2 - STOT RE 2 Inhalation - STOT SE 3
• Target Organs: Central nervous system
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 3
• Flash Point(F): 39.2 °F - closed cup
• Flash Point(C): 4 °C - closed cup