Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

GE17-0756-05

Glutathione Sepharose 4B

Cytiva 17-0756-05, pack of 100 mL

Synonym(s):

affinity chromatography resin, GST-tagged proteins purification

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

ligand

glutathione

packaging

pack of 100 mL

manufacturer/tradename

Cytiva 17-0756-05

matrix

4% agarose

particle size

45-165 μm

average diameter

90 μm

cleaning in place

4-13

working range

4-13

capacity

>25 mg binding capacity (horse liver GST/ml medium)

General description

Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.
Glutathione Sepharose 4B is designed for high capacity single-step purification of Glutathione S-Transferase (GST) tagged fusion proteins.

GST-tagged proteins and other glutathione S-transferases or glutathone-dependent proteins can be purified directly from bacterial lysates using Glutathione Sepharose 4B. Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity.

Features and Benefits

  • High capacity single step purification of recombinant glutathione S-transferase (GST) tagged proteins
  • Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity and functionality.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Storage Class Code

3 - Flammable liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Sajjan Koirala et al.
Cell reports, 32(3), 107922-107922 (2020-07-23)
Spatiotemporal control of Wnt/β-catenin signaling is critical for organism development and homeostasis. The poly-(ADP)-ribose polymerase Tankyrase (TNKS1) promotes Wnt/β-catenin signaling through PARylation-mediated degradation of AXIN1, a component of the β-catenin destruction complex. Although Wnt/β-catenin is a niche-restricted signaling program, tissue-specific

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service